Multi-host Bacterial Pathogenesis

and Anti-Infective Agents.

Laurence Rahme, Ph.D.

Associate Professor of Surgery

Microbiology and Immunobiology

Harvard Medical School

(617)724-5003 Fax:(617)724-8558

email: rahme at molbio.mgh.harvard.edu

Department of Surgery

Director of Molecular Surgical Lab

50 Blossom Street

Thier Research Bldg. Room 340

Massachusetts General Hospital

Boston, MA 02114

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Contents:

Project Overviews

Molecular Pathogenesis of Pseudomonas aruginosa

The "copper rust" blue-green opportunistic pathogen.

Cystic Fibrosis Lung Infections

P. aeruginosa infection of CF patients.

Anti-infective Chemicals

Novel anti-infective compounds to combat bacterial pathogens.

Burn Trauma

Host Response to Injury and burn wound infection

Drosophila Infection Model

P. aeruginosa PA14 is pathogenic in Drosophila.

Plant Infection Model

P. aeruginosa PA14 is pathogenic in Arabidopsis.

Affiliations

HMS Microbiology and Immunology

We are microbiologists.

Shriners Hospitals for Children

Our research will help children burn victims.

Molecular Biology at MGH

We are molecular biologists.

Division of Infectious Diseases at MGH

We study infectious disease at lung, wound and others.

Microbial Science at Harvard

We study bacterial pathogenesis.

Chemical Biology

We study anti-infective chemical compounds.

The MGH Burn Research Center

We study burn trauma and infection of burn wound.

Protocols

Drosophila Infection

Fruit flies are a good model system.

Lambda Red recombinase system

For mutagenesis of Pseudomonas aeruginosa.

Arabidopsis Infection

We also use plants to study host-microbe interactions.

Pseudomonas aeruginosa

We have a list of P. aeruginosa protocols.

Genomic Tools

GuiGraph for visualizing gene expression results.

Other Lab Protocols

For our lab members and people who are interested.