CIC End Probes on Chromosome 2

End probes from the CIC YAC library were made using a protocol developed from the IPCR procedure of Ochman et. al (1988). Table 1 below lists the combination of YAC end, restriction enzyme, and primer pair required for a given end probe amplification. Table 2 indicates the restriction enzyme used to generate each of the CIC end probes used in constructing the chromosome 2 physical map.

Table 1: End, Enzyme and Primers

       Restriction
End      Enzyme     Primers
   
Left   HindIII      1, 7
       ClaI         1, 7
       EcoRV        1, 6
   
Right  HindIII      3, 5
       Cla          3, 9
       DraI         3, 8
       PstI         3, 4
   
Primers
1  5'-gccaagttggtttaaggcgc-3'
3  5'-gcccgatctcaagattacg-3'
4  5'-ggaagaacgaaggaaggagc-3'
5  5'-aaactcaacgagctggacgc-3'
6  5'-catagtgactggcgatgctg-3'
7  5'-tcatacacggtgcctgactg-3'
8  5'-gactagctagcttagtcatt-3'
9  5'-cgtttcttggagctgggac-3'
   
   

Table 2: YAC Ends and Enzyme for Probe Preparation

CIC2A10_LE	EcoRV	Figure B
CIC2A10_RE	HindIII	Figure B
CIC2E12_LE	EcoRV	Figure D
CIC2E5_LE	HindIII	Figure F
CIC2E5_RE	HindIII	Figure F
CIC2F4_LE	EcoRV	Figure A
CIC2F4_RE	PstI	Figure A
CIC2G1_LE	HindIII	Figure A
CIC2G1_RE	HindIII	Figure A
CIC2G5_RE	HindIII	Figure A
CIC2G9_LE	HindIII	Figure E
CIC3E6_LE	HindIII	Figure B
CIC3H8_LE	HindIII	Figure D
CIC4G9_RE	HindIII	Figure B
CIC5E11_LE	HindIII	Figure F
CIC5E11_RE	HindIII	Figure F
CIC6C3_LE	HindIII	Figure F
CIC6C3_RE	PstI	Figure F
CIC6E8_LE	HindIII	Figure C
CIC6E8_RE	PstI	Figure B
CIC6F9_LE	ClaI	Figure C
CIC6G2_LE	ClaI	Figure A
CIC6G2_RE	HindIII	Figure A
CIC6G3_LE	HindIII	Figure A
CIC6G3_RE	PstI	Figure A
CIC7B5_LE	HindIII	Figure D
CIC7B5_RE	HindIII	Figure D
CIC7C11_LE	HindIII	Figure A
CIC7E10_LE	HindIII	Figure D
CIC7E2_LE	HindIII	Figure C
CIC8A1_RE	HindIII	Figure B
CIC8B10_LE	HindIII	Figure B
CIC8B10_RE	HindIII	Figure B
CIC8H12_LE	HindIII	Figure D
CIC8H12_RE	HindIII	Figure C
CIC9A3_RE	HindIII	Figure A
CIC9A4_LE	HindIII	Figure A
CIC9D8_LE	EcoRV	Figure A
CIC9D8_RE	HindIII	Figure A
CIC9H2_RE	HindIII	Figure D
CIC10A12_LE	HindIII	Figure E
CIC10A6_LE	HindIII	Figure E
CIC10F12_LE	HindIII	Figure E
CIC10F1_RE	PstI	Figure D
CIC10F4_RE	HindIII	Figure A
CIC10F7_LE	EcoRV	Figure D
CIC10F7_RE	PstI	Figure D
CIC10H6_LE	HindIII	Figure F
CIC10H6_RE	HindIII	Figure F
CIC11A4_RE	HindIII	Figure A

CIC11C11_LE	EcoRV	Figure C
CIC11C8_LE	HindIII	Figure E
CIC11C8_RE	PstI	Figure E
CIC12B1_RE	HindIII	Figure D
CIC12C10_LE	EcoR	Figure B
CIC12C10_RE	HindIII	Figure B
CIC12E3_LE	HindIII	Figure B
CIC12E3_RE	HindIII	Figure A
CIC12G5_RE	HindIII	Figure E
CIC12G8_RE	HindIII	Figure A
CIC12G9_LE	EcoRV	Figure E

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