Proc Natl Acad Sci U S A 1993 Nov 1;90(21):10370-4
Molecular Genetics Department, Cambridge Laboratory, John Innes
Centre, Norwich, United Kingdom.
A two-component transposon system based on the Ac element of
maize was used as a mutagen in Arabidopsis thaliana. Transposition
of a Ds element marked with a hygromycin-resistance gene was
activated from four different locations in the Arabidopsis genome.
The progeny of 201 plants carrying independent transposition
events were screened for mutants with severe, visible phenotypes.
Seven mutants were identified and four of them were analyzed
genetically. Three mutations were shown to be very closely linked
to a transposed copy of the element. Moreover, a mutation (alb3)
causing an albino phenotype was conclusively shown to be caused by
insertion of the Ds element: somatic and germinal reversion of the
mutation occurred in the presence of the transposase gene but not
in its absence, and in three revertants the Ds had excised from
its position in the mutant line. The DNA adjacent to Ds in the
mutant was isolated and it was demonstrated that revertants
retained part of the 8-bp duplication caused by insertion of Ds.
These experiments indicate that the Ac/Ds system can be used as an
insertional mutagen in the heterologous host Arabidopsis, which
will permit the isolation of genes from this species by transposon
PMID: 8234300, UI: 94052164