Proc Natl Acad Sci U S A 1990 May;87(10):3821-5

 

Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana.

 

Ma H, Yanofsky MF, Meyerowitz EM

Division of Biology, California Institute of Technology, Pasadena 91125.

We have isolated a gene coding for a G protein alpha subunit from the flowering plant Arabidopsis thaliana. This gene, named GPA1, was isolated by using a DNA probe generated by polymerase chain reaction based on protein sequences from mammalian and yeast G protein alpha subunits. The sequences of genomic and cDNA clones indicate that GPA1 has 14 exons, and the deduced amino acid sequence shows that the GPA1 gene product (GP alpha 1) has 383 amino acid residues (44,582 Da). The GP alpha 1 protein exhibits similarity to all known G protein alpha subunits--36% of its amino acids are identical and 73% are similar (identical and conservative changes) to mammalian inhibitory guanine nucleotide-binding regulatory factor alpha subunits and transducins. Furthermore, the GP alpha 1 protein has all of the consensus regions for a GTP-binding protein. The GPA1-encoded mRNA of 1.55 kilobases is most abundant in vegetative plant tissues, as determined by RNA blot analysis. Restriction fragment length polymorphism mapping experiments show that GPA1 is approximately 1.2 centimorgans from the visible marker er on chromosome 2.

 

MeSH Terms:

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Comparative Study
  • G-Proteins/genetics*
  • Genes, Structural, Plant*
  • Macromolecular Systems
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Plants/genetics*
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Support, Non-U.S. Gov't
  • Support, U.S. Gov't, Non-P.H.S.

Substances:

  • Oligonucleotide Probes
  • Macromolecular Systems
  • G-Proteins

Secondary source id:

  • GENBANK/M32887

PMID: 2111018, UI: 90251649