Putative genes were identified using a combination of three gene prediction programs. "Grail" (1) was run using client server software and selecting for Arabidopsis as the DNA source. "Genescan" (2) was run with the Arabidopsis organism option and exon cutoff of 1.0. "Genefinder" (3) was run with the Arabidopsis tables developed by ESSA (European Scientists Sequencing Arabidopsis). Once the gene predictions were made, the BAC sequence was compaired to sequences deposited in Genbank using blastn and blastx. In addition, the predicted translation products were compared using blastp. Colors represent the following:

 

References

1. Xu,Y., J.R Einstein, R.J. Mural, M. Shah & E.C.Uberbacher (1994) An Improved System for Exon Recognition & Gene Modeling in Human DNA Sequences. ISMB 2: 376-384

2. Burge, C. & S. Karlin (1997) Prediction of complete gene structures in human genomic DNA. J.Mol.Biol. 268: 78-94

3. Version 084. Phil Green, personal communication.