Plant Cell 1998 Jun;10(6):1043-54

The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein.


Finkelstein RR, Wang ML, Lynch TJ, Rao S, Goodman HM

Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106, USA.

Arabidopsis abscisic acid (ABA)-insensitive abi4 mutants have pleiotropic defects in seed development, including decreased sensitivity to ABA inhibition of germination and altered seed-specific gene expression. This phenotype is consistent with a role for ABI4 in regulating seed responses to ABA and/or seed-specific signals. We isolated the ABI4 gene by positional cloning and confirmed its identity by complementation analysis. The predicted protein product shows homology to a plant-specific family of transcriptional regulators characterized by a conserved DNA binding domain, the APETALA 2 domain. The single mutant allele identified has a single base pair deletion, resulting in a frameshift that should disrupt the C-terminal half of the protein but leave the presumed DNA binding domain intact. Expression analyses showed that despite the seed-specific nature of the mutant phenotype, ABI4 expression is not seed specific.


MeSH Terms:

  • Abscisic Acid/pharmacology*
  • Amino Acid Sequence
  • Arabidopsis/metabolism
  • Arabidopsis/genetics*
  • Arabidopsis/drug effects
  • Base Sequence
  • Chromosome Mapping*
  • Gamma Rays
  • Gene Expression Regulation, Plant/physiology*
  • Gene Expression Regulation, Plant/drug effects
  • Genes, Structural, Plant
  • Genetic Complementation Test
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/chemistry
  • Homeodomain Proteins/biosynthesis*
  • Molecular Sequence Data
  • Mutagenesis
  • Nuclear Proteins/genetics
  • Nuclear Proteins/chemistry
  • Nuclear Proteins/biosynthesis*
  • Plant Proteins/biosynthesis
  • Plants, Transgenic
  • Polymorphism, Restriction Fragment Length
  • Recombination, Genetic
  • Seeds/physiology
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Support, Non-U.S. Gov't
  • Support, U.S. Gov't, Non-P.H.S.


  • Abscisic Acid
  • Plant Proteins
  • Nuclear Proteins
  • Homeodomain Proteins
  • APETALA2 protein

PMID: 9634591, UI: 98299844