Biochim Biophys Acta 1997 Sep 12;1353(3):199-202

An Arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid O-methyltransferase.


Zhang H, Wang J, Goodman HM

Department of Biological Sciences, Texas Tech University, Lubbock 79409, USA.

AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.


MeSH Terms:

  • Amino Acid Sequence
  • Arabidopsis/genetics*
  • Base Sequence
  • Cloning, Molecular
  • Gene Dosage
  • Gene Expression Regulation, Plant
  • Genes, Structural, Plant/genetics*
  • Methyltransferases/metabolism
  • Methyltransferases/genetics*
  • Molecular Sequence Data
  • Organ Specificity
  • Proteins/metabolism*
  • Recombinant Fusion Proteins
  • RNA, Messenger/analysis
  • RNA, Plant/analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Support, Non-U.S. Gov't


  • 14-3-3 protein
  • RNA, Plant
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Proteins
  • caffeate O-methyltransferase
  • Methyltransferases

Secondary source id:

  • GENBANK/U70424

PMID: 9349713, UI: 98007869