Plant Physiol 1997 Dec;115(4):1525-31

Antisense inhibition of the photosystem I antenna protein Lhca4 in Arabidopsis thaliana.

Zhang H, Goodman HM, Jansson S

Department of Biological Sciences, Texas Tech University, Lubbock 79409, USA. brahz@ttacs.ttu.edu

The function of Lhca4, a gene encoding the photosystem 1 type IV chlorophyll a/b-binding protein complex in Arabidopsis, was investigated using antisense technology. Lhca4 protein was reduced in a number of mutant lines and abolished in one. The inhibition of protein was not correlated with the inhibition of mRNA. No depletion of Lhca1 was observed, but the low-temperature fluorescence emission spectrum was drastically altered in the mutants. The emission maximum was blue-shifted by 6 nm, showing that chlorophyll molecules bound to Lhca4 are responsible for most of the long-wavelength fluorescence emission. Some mutants also showed an unexplainable delay in flowering time and an increase in seed weight.

MeSH Terms:

• Arabidopsis/metabolism*
• Arabidopsis/genetics
• Arabidopsis/drug effects
• Chlorophyll/metabolism
• Gene Expression Regulation, Plant/drug effects*
• Mutagenesis
• Oligonucleotides, Antisense/pharmacology*
• Phenotype
• Photosynthetic Reaction Center, Plant/genetics
• Photosynthetic Reaction Center, Plant/biosynthesis*
• Plant Proteins/genetics
• Plant Proteins/biosynthesis*
• Plants, Transgenic
• RNA, Messenger/biosynthesis
• Support, Non-U.S. Gov't

Substances:

• Chlorophyll
• RNA, Messenger
• Plant Proteins
• Photosynthetic Reaction Center, Plant
• Oligonucleotides, Antisense
• Lhca4 protein

PMID: 9414561, UI: 98076200