Plant Physiol 1997 Dec;115(4):1525-31

Antisense inhibition of the photosystem I antenna protein Lhca4 in Arabidopsis thaliana.


Zhang H, Goodman HM, Jansson S

Department of Biological Sciences, Texas Tech University, Lubbock 79409, USA.

The function of Lhca4, a gene encoding the photosystem 1 type IV chlorophyll a/b-binding protein complex in Arabidopsis, was investigated using antisense technology. Lhca4 protein was reduced in a number of mutant lines and abolished in one. The inhibition of protein was not correlated with the inhibition of mRNA. No depletion of Lhca1 was observed, but the low-temperature fluorescence emission spectrum was drastically altered in the mutants. The emission maximum was blue-shifted by 6 nm, showing that chlorophyll molecules bound to Lhca4 are responsible for most of the long-wavelength fluorescence emission. Some mutants also showed an unexplainable delay in flowering time and an increase in seed weight.


MeSH Terms:

  • Arabidopsis/metabolism*
  • Arabidopsis/genetics
  • Arabidopsis/drug effects
  • Chlorophyll/metabolism
  • Gene Expression Regulation, Plant/drug effects*
  • Mutagenesis
  • Oligonucleotides, Antisense/pharmacology*
  • Phenotype
  • Photosynthetic Reaction Center, Plant/genetics
  • Photosynthetic Reaction Center, Plant/biosynthesis*
  • Plant Proteins/genetics
  • Plant Proteins/biosynthesis*
  • Plants, Transgenic
  • RNA, Messenger/biosynthesis
  • Support, Non-U.S. Gov't


  • Chlorophyll
  • RNA, Messenger
  • Plant Proteins
  • Photosynthetic Reaction Center, Plant
  • Oligonucleotides, Antisense
  • Lhca4 protein

PMID: 9414561, UI: 98076200