Plant Mol Biol 1997 Aug;34(6):967-71
Department of Biological Sciences, Texas Tech University,
Lubbock 79409, USA.
An Arabidopsis 14-3-3 protein, AFT1, was used as a 'bait' in
the two-hybrid system to identify its interacting proteins. One of
the candidate proteins, APX3, was identified as a putative
peroxisomal membrane-bound ascorbate peroxidase. Ascorbate
peroxidases are important defense enzymes that protect plant cells
from oxidative stress damage. DNA blot analysis indicates that
APX3 is encoded by a single-copy gene in the Arabidopsis genome.
RNA blot analyses show that APX3 transcript levels increase
slightly in response to cold, UV light, and treatments with
hydrogen peroxide and paraquat. The activity of APX3 in
Arabidopsis may be controlled in two ways: its enzymatic activity
through protein-protein interactions and its transcription by
transcriptional or posttranscriptional regulation.
Secondary source id:
PMID: 9290648, UI: 97435981