Plant Mol Biol 1996 Dec;32(5):785-96

Expression analysis of an Arabidopsis C2H2 zinc finger protein gene.

Tague BW, Gallant P, Goodman HM

Department of Genetics, Harvard Medical School, Boston, MA, USA.

C2H2 zinc finger protein genes encode nucleic acid-binding proteins involved in the regulation of gene activity. AtZFP1 (Arabidopsis thaliana zinc finger protein 1) is one member of a small family of C2H2 zinc finger-encoding sequences previously characterized from Arabidopsis. The genomic sequence corresponding to the AtZFP1 cDNA has been determined. Molecular analysis demonstrates that AtZFP1 is a unique, intronless gene which encodes a 1100 nucleotides mRNA highly expressed in roots and stems. A construct in which 2.5 kb of AtZFP1 upstream sequences is linked to the beta-glucuronidase gene was introduced into Arabidopsis by Agrobacterium-mediated transformation of roots. Histochemical analysis of transgenic Arabidopsis carrying the AtZFP1 promoter: beta-glucuronidase fusion shows good correlation with RNA blot hybridization analysis. This transgenic line will be a useful tool for analyzing the regulation of AtZFP1 to further our understanding of its function.

MeSH Terms:

• Amino Acid Sequence
• Arabidopsis/metabolism
• Arabidopsis/genetics*
• Base Sequence
• DNA-Binding Proteins/metabolism
• DNA-Binding Proteins/genetics*
• DNA, Plant
• Gene Expression
• Genes, Plant
• Histocytochemistry
• Molecular Sequence Data
• Plant Proteins/genetics*
• Plant Proteins/biosynthesis
• Plants, Transgenic
• Support, Non-U.S. Gov't
• Support, U.S. Gov't, Non-P.H.S.
• Zinc Fingers/genetics*

Substances:

• Plant Proteins
• DNA, Plant
• DNA-Binding Proteins
• AtZEP1 protein

PMID: 8980531, UI: 97134971