Construction of an ~2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2

Ming Li Wang(1,2), Lu Huang(2), Deverie K. Bongard-Pierce (2), Stephen Belmonte(2), Eve Ann Zachgo(2), John W. Morris(2), Maureen Dolan(2) and Howard M. Goodman(1,2)

(1) Department of Genetics, Harvard Medical School and
(2) Department of Molecular Biology,
Massachusetts General Hospital,
Boston, MA 02114, USA

The Plant Journal (1997) Volume 12 pages 711-730.

Summary

A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artificial chromosome (YAC) physical map is described. An ~2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.

The long tables from the paper are provided below, for those researches who may wish to obtain the data in electronic form.

• Table 2: Bac clones identified by hybridization of YAC SIA probes to grids
• Table 3: TAMU BAC clones containing rDNA or chloroplast DNA sequences identified by hybridization to the YAC probe, EG03G01
• Table 4: Unique (non repetitive) TAMU BAC clones identified by hybridization to the SIA YAC probes